RAS BiologyУспехи современной биологии Advances in Current Biology

  • ISSN (Print) 0042-1324
  • ISSN (Online) 3034-6347

Establishing a Collection of Bacteria that Mimic Human Infectious Agents in Order to use Them for the Development of Rapid Antibiotic Resistance Testing Methods

PII
S30346347S0042132425040017-1
DOI
10.7868/S3034634725040017
Publication type
Article
Status
Published
Authors
Volume/ Edition
Volume 145 / Issue number 4
Pages
297-309
Abstract
A collection of 25 bacterial isolates has been picked up from the leaves of alfalfa , culvars Kamis and Bagryany, winter rapeseed , tulip sp., green parts of , celandine and Sarepta mustard , as well as the red poultry mite and soil nematode . There are 7 representatives of the genus , and among the isolates; 5 isolates of the genus , 2 isolates of the genus , 8 isolates of the genus , and representatives of the type of Actinomycetota: and . Identification of the taxonomic affiliation of the isolates was carried out by sequencing of complete 16S ribosomal DNA genes, which are deposited in the GenBank database. The degree of resistance of isolated isolates to five common antibiotics was studied: kanamycin (Km), ampicillin (Ap), spectinomycin (Sp), erythromycin (Em) and chloramphenicol (Cm). Strains resistant to several antibiotics, to individual antibiotics, and are not resistant to any of the tested antibiotics have been identified within each taxonomic group. This makes possible comparison of the effectiveness of rapid antibiotic resistance testing methods on several groups of bacteria.
Keywords
коллекция бактерии антибиотикорезистентность 16S рДНК моделирование инфекционных агентов
Date of publication
12.05.2025
Year of publication
2025
Number of purchasers
0
Views
34

References

  1. 1. Пушкарева В.И., Литвин В.Ю., Ермолаева С.А. Растения как резервуар и источник возбудителей пищевых инфекций // Эпидемиол. вакцинопрофил. 2012. № 2 (63). С. 10–20. https://cyberleninka.ru/article/n/rasteniya-kak-rezervuar-i-istochnik-vozbuditeley-pischevyh-infektsiy (дата обращения: 25.04.2025)
  2. 2. Adane W.D., Chandravanshi B.S., Tessema M. A novel electrochemical sensor for the detection of metronidazole residues in food samples // Chemosphere. 2024. V. 359. P. 142279. https://doi.org/10.1016/j.chemosphere.2024.142279
  3. 3. Allegranzi B., Bagheri Nejad S., Combescure C. et al. Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysis // Lancet. 2011. V. 377 (9761). P. 228–241. https://doi.org/10.1016/S0140-6736 (10)61458-4
  4. 4. Dong Y., Iniguez A.L., Ahmer B.M. Kinetics and strain specificity of rhizosphere and endophytic colonization by enteric bacteria on seedlings of Medicago sativa and Medicago truncatula // Appl. Environ. Microbiol. 2003. V. 69 (3). P. 1783–1790.
  5. 5. Hassanain W.A., Johnson C.L., Faulds K. et al. Ultrasensitive dual ELONA/SERS-RPA multiplex diagnosis of antimicrobial resistance // Anal. Chem. 2024. V. 96 (29). P. 12093–12101. https://doi.org/10.1021/acs.analchem.4c02165
  6. 6. Lane D.J. 16S/23S rRNA sequencing // Nucleic acid techniques in bacterial systematics / Eds E. Stackebrandt, M. Goodfellow. N.Y.: John Wiley and Sons, 1991. P. 115–175.
  7. 7. Lane D.J., Pace B., Olsen G.J. et al. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses // PNAS USA. 1985. V. 82 (20). P. 6955–6959. https://doi.org/10.1073/pnas.82.20.6955
  8. 8. Mulani M.S., Kamble E.E., Kumkar S.N. et al. Emerging strategies to combat ESKAPE pathogens in the era of antimicrobial resistance: a review // Front. Microbiol. 2019. V. 10. P. 539. https://doi.org/10.3389/fmicb.2019.00539
  9. 9. Nakano M., Kalsi S., Morgan H. Fast and sensitive isothermal DNA assay using microbead dielectrophoresis for detection of anti-microbial resistance genes // Biosens. Bioelectron. 2018. V. 117. P. 583–589. https://doi.org/10.1016/j.bios.2018.06.063
  10. 10. Oeschger T.M., Erickson D.C. Visible colorimetric growth indicators of Neisseria gonorrhoeae for low-cost diagnostic applications // PLoS One. 2021 V. 16 (6). P. 0252961. https://doi.org/10.1371/journal.pone.0252961
  11. 11. Pasquier E., Owolabi O.O., Powell B. et al. Assessing post-abortion care using the WHO quality of care framework for maternal and newborn health: a cross-sectional study in two African hospitals in humanitarian settings // Reprod. Health. 2024. V. 21 (1). P. 114. https://doi.org/10.1186/s12978-024-01835-9
  12. 12. Rana S., Kaur K.N., Narad P. et al. Knowledge, attitudes and practices of antimicrobial resistance awareness among healthcare workers in India: a systematic review // Front Public Health. 2024. V. 12. P. 1433430. https://doi.org/10.3389/fpubh.2024.1433430
  13. 13. Saha M., Sarkar A. Review on multiple facets of drug resistance: a rising challenge in the 21st century // J. Xenobiot. 2021. V. 11 (4). P. 197–214. https://doi.org/10.3390/jox11040013
  14. 14. Salehi B., Quispe C., Butnariu M. et al. Phytotherapy and food applications from Brassica genus // Phytother. Res. 2021. V. 35 (7). P. 3590–3609. https://doi.org/10.1002/ptr.7048
  15. 15. Swami P., Sharma A., Anand S., Gupta S. DEPIS: a combined dielectrophoresis and impedance spectroscopy platform for rapid cell viability and antimicrobial susceptibility analysis // Biosens. Bioelectron. 2021. V. 182. P. 113190. https://doi.org/10.1016/j.bios.2021.113190
  16. 16. Treichel S., Hartmann M., Rump A. et al. Evaluation of a didanosin-containing regimen including genotypic resistance testing: an open-label, multicenter study // Eur. J. Med. Res. 2003. V. 8 (9). P. 405–413.
  17. 17. Wesseling C.M.J., Martin N.I. Synergy by perturbing the gram-negative outer membrane: opening the door for gram-positive specific antibiotics // ACS Infect. Dis. 2022. V. 8 (9). P. 1731–1757. https://doi.org/10.1021/acsinfecdis.2c00193
  18. 18. Zhang Y., Fan W., Shao C. et al. Rapid determination of antibiotic resistance in Klebsiella pneumoniae by a novel antibiotic susceptibility testing method using SYBR green I and propidium iodide double staining // Front. Microbiol. 2021. V. 12. P. 650458. https://doi.org/10.3389/fmicb.2021.65045
QR
Translate

Indexing

Scopus

Scopus

Scopus

Crossref

Scopus

Higher Attestation Commission

At the Ministry of Education and Science of the Russian Federation

Scopus

Scientific Electronic Library